论文部分内容阅读
目的检测HPV-16 1311～1773位63bp重复序列插入突变的E1基因(E1/dup)在宫颈炎、宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)及宫颈癌病变组织中的分布及病毒的整合状态,探讨该突变对病毒致癌活性的影响.方法收集维吾尔族和汉族宫颈炎、CIN和宫颈癌临床组织标本215例,提取组织DNA,PCR检测E1/dup突变体以及病毒E1/E2基因的完整性.结果新疆地区215例宫颈病变组织中HPV-16 E1检出率为60.00%(129/215),宫颈炎、CIN及宫颈癌病样中HPV-16 E1的检出率分别为31.15%(19/61)、49.21%(31/63)和86.81%(79/91).在HPV-16E1阳性病样中,HPV-16 E1/dup突变率为37.21%(48/129),宫颈炎、CIN和宫颈癌病样中E1/dup突变率分别为15.79%、22.58%和48.10%,E1/dup突变率在宫颈癌中显著高于宫颈炎(P<0.05)和CIN(P<0.05).E1/dup突变率在汉族和维吾尔族宫颈癌病样中分别为40.00%(6/15)和50.00%(32/64),两者无显著性差异(P>0.05).在HPV-16 E1阳性病样中病毒整合率为51.72%(60/116),E1/dup突变病样中HPV16整合率[69.05%(29/42)]明显高于未发生E1/dup突变的病样[41.89%(31/74)](P<0.05).结论新疆地区E1/dup突变率在宫颈癌中显著高于宫颈炎及CIN,E1/dup与病毒致癌活性及病毒整合相关.
Objective To detect the distribution of E1 / dup gene of HPV16 161311 ~ 1773 inserted in 63bp repeat in cervicitis, cervical intraepithelial neoplasia (CIN) and cervical cancer and the integration of virus And investigate the effect of this mutation on the oncogenic activity of the virus.Methods A total of 215 cases of cervicitis, CIN and cervical cancer tissues from Uygur and Han nationalities were collected and the tissue DNA was extracted and the complete E1 / dup and E1 / E2 genes were detected by PCR Results The positive rate of HPV-16 E1 in 215 cases of cervical lesions in Xinjiang was 60.00% (129/215). The positive rates of HPV-16 E1 in cervicitis, CIN and cervical cancer were 31.15% 19/61), 49.21% (31/63) and 86.81% (79/91) respectively.The HPV-16 E1 / dup mutation rate was 37.21% (48/129), cervicitis, The mutation rates of E1 / dup in CIN and cervical cancer were 15.79%, 22.58% and 48.10% respectively. The mutation rate of E1 / dup in cervical cancer was significantly higher than that in cervicitis (P <0.05) and CIN (P <0.05). E1 / dup mutation rates were 40.00% (6/15) and 50.00% (32/64) respectively in Han and Uigur patients with cervical cancer, but there was no significant difference between them (P> 0.05) Positive disease-like The integration rate was 51.72% (60/116), and the HPV16 integration rate in the E1 / dup mutant was significantly higher than that in the case without the E1 / dup mutation [69.05% (29/42)] [41.89% (31/74) ) (P <0.05) .Conclusion The mutation rate of E1 / dup in Xinjiang is significantly higher than that of cervicitis and CIN in the cervical cancer, and E1 / dup is associated with the carcinogenic activity of the virus and the virus integration.