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[Objective] To determine and compare the content of aristolochic acid A in RADIX ET RHIZOMA ASARI from 12 different production areas by establishing Reversed-Phase High Performance Liquid Chromatography(RP-HPLC).[Method] The content of aristolochic acid A in the extract of RADIX ET RHIZOMA ASARI prepared by ultrasonic extraction was determined by RP-HPLC.RP-HPLC used the chromatographic column Zorbax SB-C18(4.6 mm ×150 mm,5 μm) with the detection wavelength at 316 nm.Following the injection of 5 μl of sample solution at room temperature,an elution with acetonitrile-water solution(V/V,50:50) as mobile phase was initiated at a flow rate of 1.0 ml/min.[Result] Except the RADIX ET RHIZOMA ASARI in Fengcheng,no aristolochic acid A was detected.The content of aristolochic acid A in RADIX ET RHIZOMA ASARI produced in Fengcheng showed good linear relationship within the range of 2.04 to 20.4 ng/ml.[Conclusion] This method is rapid and simple with high recovery rate and good linear relationship,sensitivity and repeatability.Thus,it could be used for the determination of aristolochic acid A content in RADIX ET RHIZOMA ASARI.And the RADIX ET RHIZOMA ASARI used in this research had relatively high quality.
[Objective] To determine and compare the content of aristolochic acid A in RADIX ET RHIZOMA ASARI from 12 different production areas by establishing Reversed-Phase High Performance Liquid Chromatography (RP-HPLC). [Method] The content of aristolochic acid A in the extract of RADIX ET RHIZOMA ASARI prepared by ultrasonic extraction was determined by RP-HPLC. RP-HPLC was used for the chromatographic column Zorbax SB-C18 (4.6 mm × 150 mm, 5 μm) with the detection wavelength at 316 nm. μl of sample solution at room temperature, an elution with acetonitrile-water solution (V / V, 50:50) as mobile phase was initiated at a flow rate of 1.0 ml / min. [Result] Except the RADIX ET RHIZOMA ASARI in Fengcheng , no aristolochic acid A was detected. The content of aristolochic acid A in RADIX ET RHIZOMA ASARI produced in Fengcheng showed good linear relationship within the range of 2.04 to 20.4 ng / ml. [Conclusion] This method is rapid and simple with high recovery rate and good linear relations hip, sensitivity and repeatability.Thus, it could be used for the determination of aristolochic acid A content in RADIX ET RHIZOMA ASARI. And the RADIX ET RHIZOMA ASARI used in this research had relatively high quality.