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为检测动物源性食品中恩诺沙星残留量,评估基于卵黄抗体的间接竞争酶联免疫吸附法检测恩诺沙星的可行性,用活性脂法将恩诺沙星同卵清蛋白偶联制备免疫原和包被抗原并用紫外光谱进行验证。用聚乙二醇-6000提取卵黄抗体。五免之后效价达到峰值1∶32 000。用间接竞争酶联免疫吸附法确定包被原质量浓度、卵黄抗体的稀释倍数和IC_(50)分别为38 ng/m L、1∶64 000和18.207 ng/m L,回归曲线方程为y=0.891 1-0.016 5x(R~2=0.990)。结果表明,制备的抗恩诺沙星卵黄抗体为进一步建立检测动物源性食品中恩诺沙星的残留的免疫方法提供参考依据。
In order to detect the residues of enrofloxacin in animal-derived foods and evaluate the feasibility of enrofloxacin-based indirect competitive enzyme-linked immunosorbent assay (ELISA), enrofloxacin and ovalbumin Immunogens and coated antigens were prepared and verified by UV spectroscopy. Egg yolk antibody was extracted with PEG-6000. After the five-free price reached a peak of 1:32 000. The concentration of yolk antibody and the IC 50 of the coating were 38 ng / m L, 1:64 000 and 18.207 ng / m L, respectively. The regression curve was y = 0.891 1-0.016 5x (R ~ 2 = 0.990). The results showed that the prepared y-enrofloxacin yolk antibody provides a reference for further establishment of the residual immunization method for detecting enrofloxacin in animal-derived foods.