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用间接酶联免疫吸附测定法检测２０对生育夫妇和２８对不孕不育夫妇血清和精浆中抗精子抗体、抗精浆免疫抑制物抗体、抗子宫内膜抗体、抗卵巢抗体，结果不孕不育男性血清和精浆中抗精子抗体阳性率（分别为２８％和３５％）与生育组间有非常显著性差异，抗精浆免疫抑制物抗体阳性率（分别为２１％和２５％）与生育组间有显著性差异．不孕不育女性血清中抗精子抗体阳性率（为２１％）与生育组间无显著性差异，抗精浆免疫抑制物抗体、抗子宫内膜抗体、抗卵巢抗体阳性率（分别为２５％，２５％，２１％）与生育组间具有显著性差异．不孕和流产夫妇血清、精浆中含有１种以上抗体阳性率为７８％，提示血清和精浆中抗生育抗体的产生与不孕不育有密切关系
The serum and seminal plasma antisperm antibody, anti-seminal plasma immunosuppressant antibody, anti-endometrial antibody and anti-ovarian antibody were detected in 20 pairs of reproductive couples and 28 pairs of infertile couples by indirect enzyme-linked immunosorbent assay The positive rates of anti-sperm antibodies (28% and 35%, respectively) in pregnant and infertile men’s serum and in seminal plasma were significantly different from those in reproductive subjects. The positive rates of anti-seminal plasma immunosuppressive antibodies (21% and 25% ) And fertility groups have significant differences. The positive rate of anti-sperm antibody (21%) in infertile women’s serum was not significantly different from that in reproduction group. The positive rates of anti-seminal plasma immunosuppressant antibody, anti-endometrial antibody and anti-ovarian antibody were 25% , 25%, 21%) and fertility groups have significant differences. Infertility and abortion couple serum, seminal plasma containing more than one antibody positive rate of 78%, suggesting that serum and seminal plasma anti-fertility antibodies and infertility are closely related