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目的:研究干细胞标志物OCT-4在肺癌组织中的表达以及临床意义。方法:收集我院2003-2006年40例肺癌手术患者石蜡组织标本,免疫组化检测组织中OCT-4的表达,取癌旁组织和10例正常肺组织作为对照,回顾40例患者的临床病理指标,并分析各项指标与OCT-4的关系。对40例患者进行随访,分析OCT-4表达与肿瘤复发率之间的关系,Westernblot分析鳞癌与腺癌中OCT-4蛋白的表达。结果:OCT-4在肺癌组织中阳性表达为37.5%,显著高于正常肺组织中OCT-4的表达(P<0.05)。OCT-4的阳性表达强度随肺癌临床分期增高而增高(P<0.05);对40例患者进行3年随访,OCT-4阳性表达病例的肿瘤复发率为53.3%,较OCT-4阴性表达病例的复发率(20.0%)高,两者有显著性差异(P<0.05)。应用Western-blot法检测癌组织与正常组织,腺癌中OCT-4蛋白的表达较鳞癌中OCT-4蛋白表达增高,经过Bandscan软件灰度扫描,两者之间有显著性差异(P=0.04)。结论:OCT-4阳性表达强度与肺癌临床分期与肿瘤复发率有关,可以为判断患者预后提供参考。
Objective: To study the expression and clinical significance of stem cell marker OCT-4 in lung cancer. Methods: Forty paraffin-embedded specimens of lung cancer patients from 2003 to 2006 in our hospital were collected. The expression of OCT-4 was detected by immunohistochemistry. The paracancer tissues and 10 normal lung tissues were taken as control. The clinical pathology Indicators, and analyze the relationship between indicators and OCT-4. Forty patients were followed up. The relationship between OCT-4 expression and tumor recurrence rate was analyzed. The expression of OCT-4 protein in squamous cell carcinoma and adenocarcinoma was analyzed by Western blot. Results: The positive expression of OCT-4 in lung cancer was 37.5%, which was significantly higher than that in normal lung tissue (P <0.05). The positive expression of OCT-4 was increased with the clinical stage of lung cancer (P <0.05). The tumor recurrence rate of OCT-4 positive cases was 53.3% after 40 years of follow-up of 40 cases, (20.0%), the difference was significant (P <0.05). The expression of OCT-4 protein in adenocarcinoma was higher than that in squamous cell carcinoma by Western-blot assay. The expression of OCT-4 protein in adenocarcinoma was significantly higher than that in squamous cell carcinoma (P = 0.04). Conclusion: The positive expression of OCT-4 and the clinical stage of lung cancer and tumor recurrence rate, which can provide a reference for judging the prognosis of patients.