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目的:探讨多西他赛节律化疗对胃癌细胞及内皮细胞生物学效应的影响。方法:采用低剂量的多西他赛持续作用于人脐静脉内皮细胞株(HUVEC)和人BGC-823胃癌细胞株144h,观察细胞增殖、凋亡和血小板反应素1(TSP-1)的表达情况。结果:多西他赛对人HUVEC较BGC-823胃癌细胞的抑制作用更强,IC50值分别为(0.06±0.005)和(1.1±0.024)nmol/L。较低IC50浓度的多西他赛作用于HUVEC引起细胞凋亡的百分比近似于较高IC50浓度的多西他赛作用于BGC-823细胞的百分比。多西他赛明显地增加了人HUVEC的TSP-1表达,为对照组的(1.78±0.18)倍,而且明显地增加了TSP-1蛋白的分泌,与对照组的100%相比,多西他赛组为(806±73)%(P<0.05),而肿瘤细胞的TSP-1表达和分泌没有增加。结论:多西他赛节律化疗优先抑制了内皮细胞的增殖和诱导内皮细胞的凋亡,其机制可能是由于多西他赛节律化疗增加了内皮细胞TSP-1的表达和分泌。
Objective: To investigate the effects of docetaxel rhythmic chemotherapy on the biological effects of gastric cancer cells and endothelial cells. Methods: The proliferation, apoptosis and the expression of TSP-1 in human gastric cancer cell line HUVEC and human BGC-823 gastric cancer cell line 144h were treated with low dose of docetaxel. Happening. RESULTS: Docetaxel had a stronger inhibitory effect on human HUVECs than BGC-823 gastric cancer cells with IC50 values of (0.06 ± 0.005) and (1.1 ± 0.024) nmol / L, respectively. The percentage of apoptosis induced by docetaxel at lower IC50 concentrations in HUVECs was similar to the percentage of docetaxel treated at higher IC50 concentrations in BGC-823 cells. Docetaxel significantly increased TSP-1 expression in human HUVECs (1.78 ± 0.18) fold in the control group and significantly increased TSP-1 protein secretion compared with 100% in the control group His race group (806 ± 73)% (P <0.05), while the tumor cells TSP-1 expression and secretion did not increase. Conclusion: Docetaxel rhythm chemotherapy preferentially inhibits the proliferation of endothelial cells and induces the apoptosis of endothelial cells. The mechanism may be that docetaxel rhythm chemotherapy increases the expression and secretion of TSP-1 in endothelial cells.