【摘 要】
：
为利用高效液相色谱法检测衍生化鸡蛋样品中的二十碳五烯酸乙酯(EPA-EE)和二十二碳六烯酸乙酯(DHA-EE),使用super C18色谱柱,考察了流动相有机相比例、流速及柱温改变对EPA-E
【机 构】
：
天津农学院动物科学与动物医学学院天津市农业动物繁育与健康养殖重点实验室,天津,300384
论文部分内容阅读
为利用高效液相色谱法检测衍生化鸡蛋样品中的二十碳五烯酸乙酯(EPA-EE)和二十二碳六烯酸乙酯(DHA-EE),使用super C18色谱柱,考察了流动相有机相比例、流速及柱温改变对EPA-EE和DHA-EE分离的影响,确定了等度洗脱、流动相为甲醇-水(95∶5,Ⅵ V)、流速1.0 mL/min、柱温30℃、检测波长220 nm的色谱条件.该方法下,EPA-EE和DHA-EE分别在线性范围内浓度和峰面积呈现良好的线性关系,相关系数r分别为0.999 2和0.999 0,采用上述方法测定了ω-溴苯乙酮衍生化的鸡蛋中混合脂肪酸,结果表明EPA-EE和DHA-EE二峰可达到基线分离.本方法快速、简单、重复性好,为鸡蛋中多不饱和脂肪酸含量的快速检测提供了思路.
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