论文部分内容阅读
目的研究AIDS患者肺组织巨细胞病毒(CMV)感染与CD4~+CD25~+调节性T淋巴细胞表达的关系。方法应用免疫组织化学方法,检测15例AIDS尸检患者肺门淋巴结中CD4~+CD25~+调节性T淋巴细胞的特异性标记物叉头/翅膀状螺旋转录因子(FOXP3)的表达,同时应用免疫组织化学和核酸原位杂交方法检测肺组织中CMV抗原及核酸的表达。结果本组病例中有8例表现为肺部CMV感染。CMV肺炎主要表现为间质性肺炎及巨细胞包涵体形成;肺部CMV感染常合并其他机会性感染;应用CMV单克隆抗体免疫组织化学及原位杂交技术可明确CMV感染诊断。CMV感染者FOXP3阳性标记指数显著高于无感染者;CMV感染者CD4~+、CD8~+淋巴细胞计数略低于无感染者,但差异无统计学意义。结论本组病例AIDS患者肺部CMV感染率较高,可能与调节性T淋巴细胞的高表达有关。
Objective To study the relationship between cytomegalovirus (CMV) infection and CD4 ~ + CD25 ~ + regulatory T lymphocytes in AIDS patients. Methods Immunohistochemistry was used to detect the expression of FOXP3, a specific marker of CD4 ~ + CD25 ~ + regulatory T lymphocytes in hilar lymph nodes of 15 AIDS autopsy patients. Histochemistry and in situ hybridization were used to detect the expression of CMV antigen and nucleic acid in lung tissue. Results Eight cases in this group showed pulmonary CMV infection. CMV pneumonia mainly manifested as interstitial pneumonia and giant cell inclusion body formation; pulmonary CMV infection often associated with other opportunistic infections; CMV monoclonal antibody immunohistochemistry and in situ hybridization can confirm the diagnosis of CMV infection. The positive labeling index of FOXP3 in CMV infected patients was significantly higher than that in non-infected patients. The counts of CD4 ~ + and CD8 ~ + lymphocytes in CMV infected patients were slightly lower than those in non-infected patients, but the difference was not statistically significant. Conclusions The infection rate of CMV in lungs of AIDS patients in this group is higher than that of control group, which may be related to the high expression of regulatory T lymphocytes.