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利用第２代抗ＨＣＶＥＬＩＳＡ试剂和ＰＣＲ技术对１８６例肝病患者进行了抗ＨＣＶ和ＨＣＶＲＮＡ检测。６３例患者（３３．８７％）抗ＨＣＶ和／或ＨＣＶＲＮＡ阳性，表明本组肝病患者存在较严重的ＨＣＶ感染。ＨＣＶ标志物（ＨＣＶｍ）阳性肝病患者较ＨＣＶｍ阴性肝病患者有更高的输血、手术、接种和拔牙等血液暴露史（Ｐ＜０．０５或Ｐ＜０．０１）。６３例ＨＣＶ感染者中，３４例抗ＨＣＶ和ＨＣＶＲＮＡ同时阳性，另有患者表现为单纯抗ＨＣＶ＋（２０／６３）或单纯ＨＣＶＲＮＡ＋（９／６３），说明抗ＨＣＶ不能有效反映ＨＣＶ病毒血症，同时检测抗ＨＣＶ和ＨＣＶＲＮＡ可更全面地反映ＨＣＶ感染状况。ＨＢＶ／ＨＣＶ重叠感染者ＨＢＶＤＮＡ和ＨＣＶＲＮＡ检测结果有４种表现形式：同时阳性、同时阴性、单纯Ｈ３ＶＤＮＡ阳性或单纯ＨＣＶＲＮＡ阳性。ＨＢＶ和ＨＣＶ的相互作用有待于进一步研究。
Anti-HCV and HCV RNA were detected in 186 patients with liver disease using the second generation anti-HCV ELISA reagent and PCR technique. Sixty-three patients (33.87%) were positive for anti-HCV and / or HCVRNA, indicating a more serious HCV infection in this group of patients with liver disease. Hepatitis C virus (HCV) positive liver disease patients had higher blood transfusions, surgeries, inoculation and tooth extraction (P <0.05 or P <0.01) than HCVm negative liver disease patients. Of 63 HCV-infected patients, 34 were positive for anti-HCV and HCV RNA, while others were anti-HCV + (20/63) or pure HCVRNA + (9/63), indicating that anti-HCV did not effectively reflect HCV viremia and that at the same time Detection of anti-HCV and HCV RNA can more fully reflect the status of HCV infection. There are 4 manifestations of HBV DNA and HCV RNA in HBV / HCV overlapping patients: positive, negative, simple H3VDNA positive or simple HCV RNA positive. Interaction between HBV and HCV needs further study.