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目的检测PTEN基因在胃癌组织中的表达,并研究其临床病理意义。方法采用逆转录聚合酶链式反应(RT-PCR)法检测PTEN在40例胃癌中的表达。结果①PTNM分期Ⅰ、Ⅱ期胃癌与对照组正常胃黏膜组织PTEN的表达有显著性差异(P<0.01);PTNM分期Ⅲ、ⅠV期和Ⅰ、Ⅱ期胃癌PTEN的表达有非常显著性差异(P<0.05);②PTEN在高、中分化腺癌和低分化胃癌阳性率表达差异具有显著性(P<0.05)。③PTEN在有淋巴结转移组和无淋巴结转移组检出阳性率差异具有显著性(P<0.05)。结论PTEN的表达与胃癌临床病理特征和生物学行为存在密切关系,PTEN基因是胃癌细胞增生活跃的重要影响因素。
Objective To detect the expression of PTEN gene in gastric carcinoma and to investigate its clinicopathological significance. Methods The expression of PTEN in 40 cases of gastric cancer was detected by reverse transcription-polymerase chain reaction (RT-PCR). Results ① There was significant difference in PTEN expression between PTNM stage Ⅰ and stage Ⅱ gastric cancer and normal gastric mucosa (P <0.01). There was significant difference between PTNM stage Ⅲ, stage ⅠV and stage Ⅰ and stage Ⅱ gastric cancer PTEN expression (P <0.05). ② The positive expression rate of PTEN in high and moderately differentiated adenocarcinoma and poorly differentiated gastric carcinoma was significantly different (P <0.05). ③ The positive rate of PTEN in lymph node metastasis group and non-lymph node metastasis group was significant (P <0.05). Conclusions The expression of PTEN is closely related to the clinicopathological features and biological behavior of gastric cancer. PTEN gene is an important factor affecting the proliferation of gastric cancer cells.