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利用高效液相色谱建立了粮食中黄曲霉毒素B1的测定方法。样品经乙腈水溶液(乙腈与水体积比为84:16)提取,提取液经多功能净化柱净化、浓缩,碘衍生试剂柱前衍生、C18色谱柱分离,荧光检测器检测,外标法定量。选择线性范围在0~5.0 ng/m L之间,结果表明线性良好,方法的检出限为0.17μg/kg。对添加高、中、低3个浓度黄曲霉毒素B1的大米样品进行加标回收实验,平均回收率为92.2%、91.0%、95.2%,变异系数为2.1%、1.7%、2.3%。
Determination of aflatoxin B1 in food by high performance liquid chromatography. The sample was extracted with acetonitrile aqueous solution (volume ratio of acetonitrile to water 84:16). The extract was purified by multi-functional purification column, concentrated, iodinated derivative pre-column derivatization, C18 column separation, fluorescence detector detection, external standard method. The linear range was selected to be between 0 and 5.0 ng / mL. The linearity was good and the limit of detection was 0.17 μg / kg. The spiked recoveries of rice samples with high, medium and low concentrations of aflatoxin B1 were 92.2%, 91.0% and 95.2%, respectively. The coefficients of variation were 2.1%, 1.7% and 2.3% respectively.