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目的：分离克隆测序胃癌高表达基因ｇｃｙｓ１８ 的部分ｃ Ｄ Ｎ Ａ． 方法：采用ｍ Ｒ Ｎ Ａ 差异展示技术从胃癌 Ｇ Ｃ７９０１ 与胃粘膜 Ｇ Ｅ Ｓ１ 细胞株中分离出一差异表达基因片段，对其命名，并进行克隆、测序及 Ｇｅｎ Ｂａｎｋ 检索；以此片段作探针与细胞株总 Ｒ Ｎ Ａ 进行打点杂交． 结果：从差示 Ｐ Ｃ Ｒ 电泳凝胶中分离出大小为４１６ ｂｐ 的差异表达片段，命名为ｇｃｙｓ１８； Ｒ Ｎ Ａ 打点杂交证实ｇｃｙｓ１８ 在 Ｇ Ｃ７９０１ 中呈高表达，在 Ｇ Ｅ Ｓ１ 中呈低表达，表明ｇｃｙｓ１８ 是真正差异表达片段；对ｇｃｙｓ１８ 进行了序列分析及 Ｇｅｎ Ｂａｎｋ 检索，示其为新序列后递交给 Ｇｅｎ Ｂａｎｋ，它的 Ｇｅｎ Ｂａｎｋ 接受号为 Ａ Ｆ０７１０５７． 结论：分离并克隆了胃癌高表达基因ｇｃｙｓ１８ 的部分ｃ Ｄ Ｎ Ａ，此基因可能与胃癌发生发展有关．
OBJECTIVE: To isolate and sequence partial c D N A gene of gastric cancer highly expressed gene gcys18. METHODS: A differentially expressed gene fragment was isolated from gastric cancer G C7901 and gastric mucosae G E S 1 cell strain by m R N A differential display technology, and was named, cloned, sequenced, and retrieved by GenBank; As a probe and cell line total R N A for spot hybridization. RESULTS: A 416 bp differentially expressed fragment was isolated from the differential P C R electrophoresis gel and named gcys18. Rn A spot hybridization confirmed the high expression of gcys18 in G C7901 in G E S . Low expression in 1 indicates that gcys18 is a truly differentially expressed fragment; sequence analysis of gcys18 and GenBank search are presented as a new sequence and submitted to Gen Bank. Its Gen Bank acceptance number is A F071057 . CONCLUSION: A portion of c D N A gene of gcys18, a highly expressed gastric cancer gene, was isolated and cloned. This gene may be involved in the development of gastric cancer.