论文部分内容阅读
目的探讨Spiegelmer NOX 1255对促黄体激素释放激素-绿脓杆菌外毒素40(Luteinizing hormone-releasing hor-mone-Pseudomonas aeruginosa exotoxin 40,LHRH-PE40)与HeLa细胞上促黄体激素释放激素(LHRH)受体结合的抑制作用。方法分别将25μmol/L的LHRH-PE40、PEA和PE40与HeLa细胞共孵育24 h后,观察细胞的形态学变化;用Spiegelmer NOX1255特异性结合LHRH-PE40上的LHRH,光学显微镜观察Spiegelmer NOX 1255对LHRH-PE40的抑制作用,ELISA检测Spiege-lmer NOX 1255对LHRH-PE40与LHRH受体结合的抑制作用。结果 LHRH-PE40和PEA可直接杀伤HeLa细胞,而PE40不能对HeLa细胞产生毒性作用;Spiegelmer NOX 1255能够结合LHRH-PE40中的LHRH,抑制LHRH-PE40与LHRH受体的结合及PE40的毒性作用。结论 LHRH-PE40对HeLa细胞的毒性作用是通过细胞表面的LHRH受体产生的;Spiegelmer NOX 1255可阻断LHRH-PE40与HeLa细胞表面LHRH受体的结合。
Objective To investigate the effects of Spiegelmer NOX 1255 on the expression of luteinizing hormone-releasing hormone-releasing-hormone-releasing hormone (LHRH-PE40) and LHRH receptor on HeLa cells Inhibition of binding. Methods The morphological changes of HeLa cells were observed after incubated with 25μmol / L LHRH-PE40, PEA and PE40 for 24 hours respectively. Spiegelmer NOX1255 specific binding to LHRH on LHRH-PE40 was used to observe the effect of Spiegelmer NOX 1255 Inhibition of LHRH-PE40 and inhibition of LHRH-PE40 binding to LHRH receptor by Spiege-lmer NOX 1255 were detected by ELISA. Results LHRH-PE40 and PEA could directly kill HeLa cells, whereas PE40 did not produce toxic effects on HeLa cells. Spiegelmer NOX 1255 could bind LHRH in LHRH-PE40 and inhibit the binding of LHRH-PE40 to LHRH receptor and the toxicity of PE40. Conclusion The cytotoxic effect of LHRH-PE40 on HeLa cells is through LHRH receptors on the cell surface. Spiegelmer NOX 1255 blocks LHRH-PE40 binding to LHRH receptors on HeLa cells.