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目的:探讨灵芝三萜类化合物(GLT)对阿尔茨海默病(AD)的防治作用及其机制。方法:将自然衰老模型大鼠60只随机均分为6组,模型组(0.9%氯化钠溶液10mL/kg)、GLT低、中、高剂量组(GLT 0.25、0.5、1.0g/kg)、阳性对照组(健脑胶囊0.38g/kg)及溶媒对照组(食用油10mL/kg);5月龄大鼠10只为正常对照组(0.9%氯化钠溶液10mL/kg)。连续灌胃给药60d后,Morris水迷宫检测大鼠学习记忆能力;脑组织光化学法检测脑组织总抗氧化能力(T-AOC活性)。结果:自然衰老模型大鼠逃避潜伏期延长,搜索距离增加,探索时间及探索距离百分比减少,显示学习记忆障碍,脑组织T-AOC活性降低,GLT灌胃60d后,学习记忆成绩明显提高,T-AOC活性升高。结论:GLT对AD有防治作用,其作用机制可能和提高脑内T-AOC活性有关。
Objective: To investigate the preventive and therapeutic effects of ganoderma lucidum triterpenoids (GLT) on Alzheimer’s disease (AD) and its mechanism. Methods: Sixty rats were randomly divided into six groups: model group (0.9% sodium chloride solution 10 mL / kg), GLT low, medium and high dose groups (GLT 0.25, 0.5 and 1.0 g / kg) , Positive control group (0.38g / kg) and vehicle control group (edible oil 10mL / kg); 10-month-old rats were normal control group (0.9% sodium chloride solution 10mL / kg). Sixty days after continuous intragastric administration, the learning and memory abilities of rats were detected by Morris water maze and the total antioxidant capacity (T-AOC activity) of brain tissue was determined by photochemical method. Results: The natural aging model rats prolonged escape latency, increased search distance, exploration time and exploration distance percentage decreased, indicating that learning and memory impairment, brain T-AOC activity decreased, GLD significantly increased after 60 days of learning and memory performance, T- AOC activity increased. Conclusion: GLT can prevent and treat AD. Its mechanism may be related to the increase of T-AOC activity in the brain.