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本文研究了Rh(D)阴性个体被Rh(D)阳性红细胞免疫后的免疫回忆反应。研究对象为11例Rh(D)阴性妇女,既往妊娠至少有一次患新生儿溶血病的历史及分娩前后间接抗人球蛋白试验阳性。对照组为11例Rh(D)阴性但未被Rh(D)阳性红细胞免疫的男性,以及11例Rh(D)阳性而未被任何血型系统抗原免疫者。方法:静脉采血20ml,肝素抗凝,分离单个核细胞(主要为淋巴细胞),以RPMI 1640洗涤后作细胞培养,随后加入Rh(D)阳性或Rh(D)阴性的O型红细胞基质,再培养5天后加入~3H胸腺嘧啶核甙,继续培养18小时后,先以4℃生理盐水洗涤,再以10%冷三氯醋酸(TCA)处理,然后收集TCA,测定所培养细胞结合的~3H胸腺嘧啶核甙量,结果按Gold
In this paper, immune response to Rh (D) -negative individuals immunized with Rh (D) -positive erythrocytes was studied. The subjects were 11 Rh (D) -negative women who had a history of hemolytic disease at least once in their previous pregnancies and an indirect anti-human globulin test before and after childbirth. The control group consisted of 11 males with Rh (D) -negative but not Rh (D) -positive erythrocytes and 11 Rh (D) -positive individuals who were not immunized with any of the blood group system antigens. Methods: Venous blood was collected in 20 ml and anticoagulated with heparin. Mononuclear cells (mainly lymphocytes) were isolated and washed with RPMI 1640 for cell culture followed by addition of Rh (D) -positive or Rh (D) -negative O-type erythrocyte matrix 3H thymidine was added 5 days after the culture, and after 18 hours of further culture, the cells were washed with physiological saline at a temperature of 4 ° C. and then treated with 10% cold trichloroacetic acid (TCA), then the TCA was collected, and the bound 3H Thymidine amount, the results by Gold