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The isomerization of light paraffin over HZSM-5-Ni-Mo-F synthetic zeolite catalysts was studied, and the effects of reaction conditions on the isomerization were investigated. The results show that the optimum reaction condition can be obtained to enhance the research octane number (RON) of product and the liquid yield. The optimum experimental condition is: HZSM-5 catalyst with 1.5wt% of Ni, 2wt% of Mo and 0.4wt% ofF, at a temperature of 345℃and a reaction mass hourly space velocity (MHSV) of 0.2 h-1. The isomerization reaction of light paraffin from Tarim refinery was studied and the research octane number (RON) of gasoline product could be enhanced by 20 units under the condition of nonhydrogenization and optimum experimental status.