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本文应用焦锑酸分子探针、X射线显微分析及图像分析方法,对不同缺血时相再灌注时心肌细胞线粒体的体积密度及其内含物性质和含量进行了定量分析。结果表明:线粒体内含物为含钙的化台物,线粒体及其钙盐颗粒的体积密度随着缺血时间的延长而逐渐增加,并被再灌注所加重,尤以缺血40min再灌注组最明显,而缺血60min再灌注组线粒体钙盐颗粒的体积密度较缺血40min再灌注组明显减少,这提示不同缺血时间的心肌对再灌注的反应有一定的规律性,是由可逆向不可逆转变的过程,在本实验条件下,缺血40min至60min可能是可逆性与不可逆性再灌注损伤的临界点。应用丹参制剂能明显减少钙盐颗粒的体积密度,提示丹参对心肌“钙超载”呈明显的保护作用,具有良好的钙拮抗效应。
In this paper, we used quantitatively labeled antimoniate molecular probes, X-ray microanalysis and image analysis methods to quantitatively analyze the bulk density of mitochondria and the contents and contents of mitochondria during reperfusion after ischemia. The results showed that the contents of mitochondria were calcium-containing compounds, and the bulk density of mitochondria and its calcium salt particles increased gradually with the prolongation of ischemia, which was further aggravated by reperfusion, especially in the reperfusion group The most obvious is that the bulk density of mitochondrial calcium salt particles in ischemia-reperfusion group was significantly lower than that in ischemia-reperfusion group at 40min reperfusion, which suggested that the myocardial response to reperfusion in different ischemic time had some regularity, Irreversible change process, in this experimental conditions, ischemia 40min to 60min may be reversible and irreversible reperfusion injury of the critical point. Salvia miltiorrhiza preparation can significantly reduce the bulk density of calcium salt particles, suggesting that Salvia miltiorrhiza on myocardial “calcium overload” was significantly protective effect, has a good calcium antagonistic effect.