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为确定纤维素酸解副产物对C.acetobutylicum CICC8012发酵生产纤维燃料丁醇的影响,选择乙酸根、甲酸、葡萄糖醛酸、氯化钠、硫酸钠、糠醛、羟甲基糠醛(HMF)、香豆酸、阿魏酸、丁香醛、香草醛等纤维素酸解副产物,研究其对菌种发酵的影响.结果表明:甲酸和酚类物质的抑制作用强烈,0.5 g/L的甲酸、阿魏酸或香豆酸存在时对丁醇的抑制率分别为88.1%、22.1%、82.4%,丁香醛(≥0.5 g/L)、香草醛(≥2 g/L)可使发酵的延滞期变长,但抑制作用小于阿魏酸和香豆酸;1 g/L的糠醛、HMF、葡萄糖醛酸存在时对丁醇的抑制率分别为9.8%、4.6%、7.3%,10 g/L乙酸钠存在时对丁醇的抑制率为4.8%,这4种物质对丁醇发酵的抑制作用不大;硫酸钠、氯化钠质量浓度大于5 g/L时对发酵产生抑制作用,浓度达到20 g/L时菌体几乎不能生长.当各抑制物混合时,若抑制物的含量超过临界点,各抑制物间存在相互促进的作用;小麦秸秆水解液的发酵结果表明,水解液成分复杂,抑制作用更为强烈.
To determine the effect of cellulose byproducts on the fermentation of butanol by fermentation of C. acetobutylicum CICC 8012, acetate, formic acid, glucuronic acid, sodium chloride, sodium sulfate, furfural, HMF, Acid, ferulic acid, syringaldehyde, vanillin and other cellulose acid by-products were studied to study their effects on the fermentation.The results showed that the inhibitory effect of formic acid and phenolic compounds was very strong, with 0.5 g / L formic acid, The inhibitory rates of butanol on the basis of 88.1%, 22.1%, 82.4%, respectively, in the presence of ferulic acid or coumaric acid, syringaldehyde (≥0.5 g / L) and vanillin (≥2 g / L) But the inhibitory effect was less than that of ferulic acid and coumaric acid. The inhibitory rates of butanol with 1 g / L furfural, HMF and glucuronic acid were 9.8%, 4.6%, 7.3% and 10 g / L respectively The inhibitory rate of butanol to sodium in the presence of sodium was 4.8%. These four substances had little inhibitory effect on the fermentation of butanol. When sodium sulfate and sodium chloride were more than 5 g / L, the inhibitory effect on the fermentation was 20% g / L almost no growth of bacteria when each inhibitor mixed, if the inhibitor content exceeds the critical point, there is mutual inhibition between the inhibitors Effect; fermentation results showed that wheat stalk hydrolyzate, hydrolyzate complex components, stronger inhibition.