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目的:建立冬菊利咽合剂中绿原酸含量测定的高效液相色谱方法。方法:采用色谱柱为Kromasil C_(18)柱(250mm×4.6mm,5μm),以乙腈-0.4%磷酸溶液(10:90)为流动相,流速为1.0ml·min~(-1),检测波长为327nm进行检测。结果:绿原酸在16.64～37.44μg·ml~(-1)范围内线性关系良好(r=0.999 8),平均回收率为99.2%,RSD为0.94%(n=6)。结论:该方法操作简便、准确、精密度高,可用于控制冬菊利咽合剂的质量。
Objective: To establish HPLC method for determination of chlorogenic acid in Dongju Liyan mixture. Methods: The chromatographic column was Kromasil C 18 column (250mm×4.6mm, 5μm) with acetonitrile-0.4% phosphoric acid solution (10:90) as the mobile phase and the flow rate was 1.0ml·min -1. The wavelength is 327nm for detection. Results: The linearity of chlorogenic acid was in the range of 16.64～37.44 μg·ml -1 (r=0.999 8). The average recovery was 99.2% and the RSD was 0.94% (n=6). Conclusion: The method is simple, accurate, accurate and can be used to control the quality of Dongju Liyan Mixture.