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目的研究土壤养分因子对鸡血藤黄酮类成分含量的影响。方法紫外-可见分光光度法测定鸡血藤中总黄酮的含量,HPLC法测定儿茶素与表儿茶素的含量,常规方法测定土壤养分的含量;应用SPSS17.0统计软件,对不同居群鸡血藤中黄酮类成分含量和土壤养分含量进行统计分析,采用主成分分析法提取土壤养分主成分,多变量线性逐步回归法建立土壤养分因子与黄酮类成分含量之间的多元回归方程。结果有效铁对总黄酮含量有促进作用;有效锰、全钾、速效钾等对黄酮含量呈抑制作用,其中有效锰作用最明显,全钾和速效钾次之。速效磷、土壤p H、有效锌、有效钙对儿茶素含量均有明显的促进作用;有效硼则呈明显的抑制作用。速效磷对表儿茶素含量呈明显促进作用,土壤p H和有效钙的促进作用次之;有效硼则呈明显抑制作用,全磷、有效铜抑制作用次之。结论土壤养分中的有效铁、有效锌、速效磷、有效锰、土壤p H、有效硼、有效钙对鸡血藤中黄酮类成分含量有明显的影响。
Objective To study the effect of soil nutrient factors on the flavonoids content of spatholobus. Methods The content of total flavonoids in Millettia radix is measured by ultraviolet-visible spectrophotometry, the content of catechins and epicatechin were determined by HPLC, and the content of soil nutrients was determined by routine methods. By using SPSS17.0 statistical software, The content of flavonoids and the content of soil nutrients in Millettia were statistical analyzed. Principal component analysis (PCA) was used to extract the main components of soil nutrients. Multivariate linear stepwise regression was used to establish the multiple regression equation between soil nutrients and flavonoids content. Results Available iron promoted the content of total flavonoids. Available manganese, total potassium and available potassium inhibited the content of flavonoids. Among them, the effective manganese was the most obvious, followed by potassium and available potassium. Available phosphorus, soil p H, available zinc and available calcium had a significant promoting effect on the content of catechins; while available boron showed a significant inhibitory effect. Available phosphorus had a significant role in promoting epicatechin, followed by p H and available Ca in soil. Available boron showed a significant inhibitory effect, followed by total phosphorus and available copper. Conclusion Available iron, available zinc, available phosphorus, available manganese, soil p H, available boron and available calcium in soil nutrients have a significant effect on flavonoids content in Millettia.