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目的:制备呋甾皂苷的生物转化产物。方法:利用米曲霉对原薯蓣皂苷(化合物1)进行转化,转化产物用大孔吸附树脂柱色谱,C18反相硅胶柱色谱,硅胶柱色谱以及高效液相色谱等进行分离纯化,通过光谱分析(FAB-MS,1HNMR,13CNMR)鉴定化学结构。结果:得到了5个转化产物,包括3个呋甾皂苷和2个螺甾皂苷。结论:米曲霉可以在保留原薯蓣皂苷的26-位葡萄糖的情况下水解3-位末端1→2鼠李糖生成次生呋甾皂苷(化合物4)。米曲霉也可以去掉原薯蓣皂苷的22-位羟基生成20(22)双键的呋甾皂苷产物(化合物3和5),并且继续水解26-位葡萄糖生成相应的螺甾皂苷(化合物2和6)。
Purpose: To prepare biotransformation products of furostatins. Methods: The original diosgenin (compound 1) was transformed by Aspergillus oryzae. The products were isolated and purified by macroporous resin column chromatography, C18 reverse phase silica gel column, silica gel column chromatography and high performance liquid chromatography (HPLC) FAB-MS, 1 HNMR, 13CNMR) to identify the chemical structure. Results: Five transformation products were obtained, including three furoside and two spiroside. CONCLUSION: Aspergillus oryzae can hydrolyze the 3-terminal 1 → 2 rhamnose to produce the secondary furostatins (compound 4) while retaining the 26-position glucose of the original diosgenin. Aspergillus oryzae can also remove the furoseoside product of the 20 (22) double bond from the 22-hydroxyl group of the original diosgenin (compounds 3 and 5) and continue hydrolysis of the 26-position glucose to the corresponding spiroside (compounds 2 and 6 ).