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以延边地区对磺酰脲类除草剂具抗、感性的慈姑为材料,采用RACE方法克隆了抗、感性慈姑的乙酰乳酸合酶(Acetolactate synthase,ALS)基因全长,分析了对除草剂抗、感性慈姑ALS基因c DNA编码区域的碱基差异,找出ALS基因的突变位点,为阐明慈姑对磺酰脲类除草剂抗性的突变机理提供分子依据。结果表明:与感性相比延边地区抗除草剂慈姑ALS基因共发生了7个碱基位点的突变,除了保守区域内第324位的苏氨酸(T3 24)突变为丙氨酸(A3 24)外,还包括非保守区域第17位的苏氨酸(T17)突变为脯氨酸(P17),而其他5个位点是氨基酸未改变的同义突变。这种错义突变和同义突变可能是延边地区抗性慈姑响应环境胁迫的分子基础。
Using the resistant and susceptible arrowheads of sulfonylurea herbicide in Yanbian area as materials, the full-length cDNA of Acetolactate synthase (ALS) gene was cloned by RACE method. The effects of herbicide resistance, In order to elucidate the mechanism of the mutation in the resistance of sulfamethoxazole to sulfonylurea herbicide, we found that the mutation of ALS gene in Alzheimer ’s disease caused by sulfaurea herbicide. The results showed that there was a 7-bp mutation in the ALS gene from Herba Alliguanus in Yanbian, except that the 324th threonine (T3 24) in the conserved region was changed to alanine (A3 24 ), The threonine (T17) in the non-conserved region at position 17 is mutated to proline (P17), while the other 5 sites are synonymous mutations in which the amino acids have not changed. This missense mutation and synonymous mutation may be the molecular basis of resistance to environmental stress in Yanbian area.