由于扫描电镜能立体观察组织细胞表面结构 ,现已广泛应用于耳蜗组织的研究。但扫描电镜只能从细胞顶部观察 ,难以立体地全面研究其细胞表面结构。本文试图研究一种能扫描单个毛细胞的方法。1　材料与方法1 .1 　 实验动物Sprague Dawley(SD)大鼠 5只 ,2周龄 ,雌雄兼用 ,由上海 Scanning electron microscopy can be three-dimensional observation of tissue cell surface structure, has been widely used in cochlear tissue research. However, SEM can only observe from the top of the cell, so it is difficult to comprehensively study the cell surface structure in three dimensions. This article attempts to study a method that can scan individual hair cells. 1 Materials and methods 1.1 Experimental animals Sprague Dawley (SD) rats 5, 2 weeks old, both male and female, from Shanghai