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目的:探讨加味玉屏风散对创伤应激小鼠脾淋巴细胞IL-2与IL-2R基因表达的影响。方法:应用体外细胞培养和Northern印迹法图像分析仪定量检测了加味玉屏风散对刀豆蛋白A(ConA)诱导的小鼠脾淋巴细胞IL-2与IL-2R基因表达的影响。结果:发现加味玉屏风散体内应用对创伤应激小鼠IL-2mRNA及IL-2RmRNA水平均具有明显的升高作用。结论:加味玉屏风散可通过促进创伤应激小鼠脾淋巴细胞IL-2及IL-2R的基因转录表达进而逆转IL-2及IL-2R的抑制状态。
Objective: To investigate the effect of Jiawei Yupingfeng Powder on IL-2 and IL-2R gene expression in splenic lymphocytes of mice with traumatic stress. METHODS: The effect of Jiawei Yupingfeng Powder on concanavalin A (ConA)-induced IL-2 and IL-2R gene expression in mouse spleen lymphocytes was quantitatively detected by in vitro cell culture and Northern blot image analysis. Results: It was found that the application of modified Yupingfeng Powder can significantly increase the levels of IL-2 mRNA and IL-2R mRNA in wound-stress mice. Conclusion: Jiawei Yupingfeng Powder could reverse the inhibitory state of IL-2 and IL-2R by promoting transcriptional expression of IL-2 and IL-2R genes in splenic lymphocytes of mice with traumatic stress.