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目的 研究丹参对大鼠肝脏低温保存损伤的保护作用。方法 采用大鼠肝脏离体非循环灌注模型 ,观察乳酸林格液 (LR液 )中加入不同剂量丹参后保存大鼠肝脏 1 2h的效果。结果 丹参组 ( 60 0mg/g)肝组织ATP含量 ( 6.0 8± 0 .67) μmol/g及分泌胆汁量 ( 1 0 5.6± 1 2 .4) μl/h明显高于对照组 ( 2 .52± 0 .31 ) μmol/g及 ( 57.4± 8.2 ) μl/h(P <0 .0 5) ,丹参组 ( 60 0mg/g)肝组织 2 ,3 二羟苯甲酸 ( 2 ,3 DHBA) ( 0 .1 54± 0 .0 1 3)nmol/g、2 ,5 DHBA( 1 .354± 0 .0 68)nmol/g及流出液天门冬氨酸转氨酶 (AST) ( 38.4± 3.7)U/L明显低于对照组 ( 0 .2 4 5± 0 .0 2 1 )nmol/g、( 2 .1 0 5± 0 .0 97)nmol/g及( 76.4± 9.2 )U/L ,差异有显著性 (P <0 .0 5)。结论 丹参可明显提高供体肝脏的保存效果 ,其作用机理可能主要与改善低温保存肝脏的能量代谢及抗氧自由基的作用有关
Objective To study the protective effect of radix salviae miltiorrhizae on rat liver hepatic cryopreservation. Methods Rat hepatic non-circulatory perfusion model was used to observe the effect of adding different doses of Salvia miltiorrhiza on the livers of rats for 12 hours after the Ringer’s solution (LR solution). Results The ATP content (6.0 8 ± 0.67) μmol / g and the amount of secreted bile (105.6 ± 122.4) μl / h in the liver tissue of Salvia miltiorrhiza group (60 mg / g) were significantly higher than those of the control group (P <0. 05) in the Salvia miltiorrhiza group (60.0 mg / g), 2,3-dihydroxybenzoic acid (2, 3 DHBA) (1.54 ± 0.068) nmol / g and the effluent aspartate aminotransferase (AST) (38.4 ± 3.7) U / L was significantly lower than that of the control group (0.24 ± 0.021 nmol / g, (2.0105 ± 0.097) nmol / g and (76.4 ± 9.2) U / L) Significance (P <0.05). Conclusion Salvia miltiorrhiza can significantly improve the preservation effect of the donor liver, and its mechanism of action may be mainly related to the improvement of energy metabolism and antioxidant free radical in the liver