论文部分内容阅读
背景: 表皮干细胞在创伤修复中具有重要作用,从诱导干细胞角度来阐释中药加速受损皮肤再生的机制值得研究。目的: 观察玉红膏对大鼠创伤愈合过程中皮肤组织中表皮干细胞增殖、分化的影响。设计: 随机对照动物实验。单位: 中国中医科学院望京医院药理室。材料: 实验于 2006- 07/09 在中国中医科学院望京医院药理室完成。选用114 只清洁级健康 Wistar 雄性成年大鼠,体质量 180～210 g,由中国中医科学院中医基础理论研究所实验动物中心提供。实验过程中对动物的处置符合动物伦理学标准。玉红膏购自中国中医科学院广安门医院制剂中心( 批号 20020110) , 京万红为天津达仁堂达二药业有限公司产品( 批号 Z12020440) 。方法: 按完全随机法将大鼠分为玉红膏组、京万红组、模型组,每组 36 只,另设 6 只为正常组。正常组常规饲养不作任何处理,其余各组玉红膏组造模后立即外敷玉红膏,每个创面 0.1 g; 京万红组每个创面外敷京万红0.1 g,每日定时换药 2 次至创面愈合; 模型组创面不予处理。主要观察指标: 于造模后第 1, 3, 5, 7, 14, 21 天 6 个时间点观察记录各组大鼠创面愈合时间并取创面皮缘比较各组各观测点整合素β1 吸光度值及转录因子 p63 阳性细胞数。结果: 纳入大鼠 108 只均进入结果分析。①创面愈合时间: 玉红膏组与京万红组大鼠均短于模型组,差异有统计学意义(P < 0.05) 。②整合素β1 吸光度值及转录因子 p63 表皮层阳性细胞数: 玉红膏组、京万红组及模型组在造模后两指标均高于正常组,差异有统计学意义(P < 0.05); 玉红膏组及京万红组在第 7 天达到峰值,峰值出现的时间早于模型组,峰值的强度高于模型组,差异有统计学意义(P < 0.05)。结论: 玉红膏有促进大鼠创面愈合的作用,该作用可能与其诱导创缘残留的表皮干细胞增殖分化有关。
BACKGROUND: Epidermal stem cells play an important role in wound repair. It is worth studying the mechanism of accelerating the regeneration of damaged skin from the perspective of induced stem cells. Objective: To observe the effect of Yuhong cream on the proliferation and differentiation of epidermal stem cells in rat skin during wound healing. Design: Randomized controlled animal experiment. Unit: Pharmacology Department of Wangjing Hospital, China Academy of Chinese Medical Sciences. MATERIALS: The experiment was performed at the Pharmacology Department of Wangjing Hospital, China Academy of Chinese Medical Sciences from July 2006 to September 2007. One hundred and one clean-health Wistar male adult rats weighing 180-210 g were selected and provided by the Experimental Animal Center of the Institute of Basic Theory of Chinese Medicine, China Academy of Chinese Medical Sciences. The disposal of animals during the experiment complied with animal ethical standards. Yuhong Ointment was purchased from the preparation center of the Guang’anmen Hospital of the Chinese Academy of Traditional Chinese Medicine (lot No. 20020110) and Jingwanhong was the product of the Tianjin Darentang Tat II Pharmaceutical Co., Ltd. (lot No. Z12020440). Methods: Rats were divided into Yuhong cream group, Jingwanhong group and model group by complete randomization method, 36 rats in each group and 6 rats in the normal group. The normal group did not do any treatment routinely, and the remaining groups of Yuhong paste group applied external Yuhuang cream immediately after modeling, each wound was 0.1 g; Jing Wanhong group applied 0.1 g of Jingwanhong on each wound surface, and the time was changed daily 2 The wounds healed from the second time; The wounds in the model group were not treated. MAIN OUTCOME MEASURES: The wound healing time of each group was observed and recorded at the first, third, fifth, seventh, and fifteenth days after model establishment. The integrin β1 absorbance values at each observation point in each group were compared. And transcription factor p63 positive cell number. Results: 108 rats were included in the results analysis. 1 Wound healing time: Yuhong paste group and Jingwanhong group rats were shorter than the model group, the difference was statistically significant (P < 0.05). 2 The integrin β1 absorbance value and transcription factor p63 number of epithelial cells: Yuhong cream group, Jingwanhong group and model group were higher than the normal group after modeling, the difference was statistically significant (P < 0.05). The Yuhong cream group and Jingwanhong group reached the peak on the 7th day, and the peak time was earlier than that of the model group. The peak intensity was higher than that of the model group, and the difference was statistically significant (P < 0.05). Conclusion: Yuhong cream can promote wound healing in rats, and this effect may be related to the proliferation and differentiation of epidermal stem cells that induce residual margin of the epidermis.