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该研究以兰州百合商品种球鳞片为外植体材料,通过组织培养诱导丛生芽萌发及高频增殖,再以丛生芽为材料诱导其发育形成小鳞茎,调节培养基对小鳞茎进行膨大发育培养,最终形成促进兰州百合组培鳞茎膨大发育的“三步”组培培养技术路线;对发育过程中形成的丛生芽、小鳞茎及膨大鳞茎进行淀粉含量测定与生长特征参数统计,分析各步培养对鳞茎形成发育过程中淀粉含量与形态变化的影响。结果表明:所建立的“三步”培养方案培育出的组培鳞茎直径、重量与鳞片数分别为1.66 cm、2.48 g和26.33片,有效地促进了鳞茎的膨大,并能诱导鳞茎主茎杆的形成发育;在培养进程中其淀粉含量呈现逐步增加的趋势,这表明与鳞茎膨大发育正相关,同时鳞茎大小、重量及鳞片数三者也表现为正相关性;当鳞茎所含鳞片数在26片以上时,其生长点易发育形成主茎杆。该文研究了兰州百合组培鳞茎的形成与膨大发育技术,所研发的“三步”培养组成的鳞茎膨大发育组培技术有效地促进了鳞茎的膨大发育,而膨大发育的鳞茎能有效地缩短田间生长周期,从时间上提高百合生产量,同时为实现兰州百合膨大的鳞茎种球规模化生产提供技术支撑。
In this study, Lanzhou lily bulbs were used as explants to induce bulblets to germinate and high-frequency proliferation through tissue culture, and then the bulbs were used as materials to induce bulblets to develop. Bulb bulbs were expanded and cultured by adjusting medium , Eventually forming a “three-step” tissue culture technology to promote bulblets development of Lanzhou lily tissue bulbs; statistics and analysis of starch content and growth characteristic parameters of clustered shoots, bulblets and bulblets formed during development Effects of Step Culture on Starch Content and Morphological Changes During the Bulb Formation and Development. The results showed that the diameter, weight and the number of scales of cultivated tissue culture bulbs were 1.66 cm, 2.48 g and 26.33, respectively, which were established by the “three-step” training program, which effectively promoted bulging of bulbs and induced bulblets The formation of stalk; its starch content showed a gradual increase in the course of culture, which shows that bulblet development is positively correlated, while the size of the bulbs, weight and scale number of the three also showed a positive correlation; when the bulb containing scales When the number of 26 or more, the growth point is easy to develop the formation of the main stem. In this paper, the formation and expansion of bulblets in Lanzhou lily tissue culture were studied. Bulblet development and tissue culture techniques developed by “three-step ” cultivation effectively promoted bulblet development of bulblets and bulked bulbs were effective To shorten the field growth cycle, improve lily production from time to time, at the same time in order to achieve large-scale production Lanzhou lily bulb bulbs to provide technical support.