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目的 研究维甲酸核内受体β（RAR β）转染血小板衍生生长因子（PDGF）激活的HSC后给予相应配体全反式维甲酸（ATRA）对细胞增殖、表型的影响。方法 质粒pCMV-script-RARβ转染经PDGF激活的大鼠HSC, western blot鉴定转染成功后,MTT法、BrdU掺入检测细胞生长情况,免疫细胞化学法检测α-SMA、肌间线蛋白的表达,western blot检测RARβ蛋白表达。结果 受体转染后再给予相应配体可使PDGF激活后HSC的RARβ受体表达升高至少维持144 h,并使其增殖减慢,α-SMA、肌问线蛋白表达减弱,较空载组、PDGF组、未给予配体组、无关配体组差别均有显著性意义。结论 RAR β受体基因转染并给予ATRA可使激活的HSC增殖减慢、表型逆转。
Objective To investigate the effects of retinoic acid (ATRA) on cell proliferation and phenotype after RAR β transfection with platelet-derived growth factor (PDGF) -activated HSC. Methods The transfected HSCs were transfected with PDGF by plasmid pCMV-script-RARβ. The expression of α-SMA and SMA was detected by MTT assay and BrdU incorporation assay after transfection. Expression, Western blot detection of RARβ protein expression. Results After transfection, the expression of RARβ receptor in HSC after PDGF activation was maintained for at least 144 h, and the proliferation of HSCs was slowed down. The expression of α-SMA and myogenin decreased compared with no-load Group, PDGF group, no ligand group, unrelated ligand group differences were significant. Conclusion Transfection of RAR β receptor gene and administration of ATRA can slow down the activated HSC proliferation and reverse the phenotype.