目的以肠衣生产的副产物肠黏膜为原料,进行大分子DNA的提取,并进行质量研究。方法用酶解-超滤法提取DNA,工艺过程中监控主要组分的分离及终产品质量。结果酶解法释放基因组DNA、肝素,蛋白质被水解为寡肽及氨基酸,DNA被超滤截留,钙盐沉淀法去除大分子肝素。终产品在含量及纯度上达到国外同类产品水平。结论从肠黏膜提取DNA切实可行,对猪小肠综合利用有着重要的现实意义。 Objective The intestinal mucosa, a byproduct of enteric coat production, was used as raw material to extract the macromolecular DNA and to study its quality. Methods DNA was extracted by enzymolysis - ultrafiltration, the separation of the main components and the quality of the final product were monitored in the process. Results Enzymolysis released genomic DNA, and heparin and protein were hydrolyzed into oligopeptides and amino acids. DNA was separated by ultrafiltration and calcium salt precipitation to remove the macromolecular heparin. The final product content and purity reached the level of similar foreign products. Conclusion It is practicable to extract DNA from intestinal mucosa and has important practical significance for the comprehensive utilization of pig intestine.