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用IL-3基因转染的瘤苗治疗实验性肺转移荷瘤小鼠,结果发现能使其肺转移性肿瘤结节比常规瘤苗治疗明显减少、存活期明显延长,而且当与IL-2或低剂量Cy合用后,可使荷瘤小鼠的肺转移结节更少,存活期更长,特别是当IL-3基因转移的瘤苗、IL-2、低剂量Cy三者合用时抗肿瘤转移作用最强,免疫学研究表明,经IL-3基因转染瘤苗治疗后,荷瘤小鼠脾脏CIL活性、NK活性、IL-2诱导的LAK活性均显著增强,脾细胞体外分泌IFN-γ和TNF水平显著升高;当与IL-2或低剂量Cy合用时,上述抗肿瘤免疫功能升高得更加明显,并且以三者联合应用后免疫功能增强最为明显,可见IL-3基因转染的瘤苗能有效地激活体内抗肿瘤免疫功能,具有显著的抗肿瘤作用.
Treatment of experimental lung metastasis-bearing mice with IL-3 gene-transformed tumor vaccines revealed that lung metastatic tumor nodules were significantly reduced, survival was significantly prolonged, and IL-2 was observed when compared with conventional tumor vaccine treatment. In combination with low-dose Cy, tumor-bearing mice can have fewer metastatic nodules and a longer survival period, especially when the IL-3 gene transfer tumor vaccine, IL-2, and low-dose Cy are combined. The tumor metastasis was the strongest. Immunological studies showed that after IL-3 gene transfection of tumor vaccines, the spleen CIL activity, NK activity, and IL-2-induced LAK activity of tumor-bearing mice were all significantly enhanced, and splenocytes secreted IFN in vitro. -The level of γ and TNF increased significantly; when combined with IL-2 or low dose of Cy, the above anti-tumor immune function increased more significantly, and the combination of the three showed the most obvious increase in immune function, visible IL-3 gene The transfected tumor vaccine can effectively activate the anti-tumor immune function in vivo and has a significant anti-tumor effect.