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目的 观察痛稳素碳末端八肽在小鼠脑内对孤啡肽对抗吗啡的镇痛作用的影响。方法 固相多肽合成法合成了痛稳素碳末端八肽 ,用辐射热甩尾法测定痛阈 ,观察 (1)小鼠脑室注射 (icv)孤啡肽对吗啡镇痛作用的影响 ;(2 )小鼠脑室注射 (icv)痛稳素碳末端八肽对小鼠基础痛阈的影响 ;(3)小鼠脑室联合注射 (icv)痛稳素碳末端八肽和孤啡肽对吗啡镇痛作用的影响。结果 孤啡肽可对抗吗啡的镇痛作用 ;痛稳素碳末端八肽本身不影响小鼠的基础痛阈 ,但可逆转孤啡肽对抗吗啡的镇痛作用。结论 痛稳素碳末端八肽在脊髓以上水平可以逆转孤啡肽对抗吗啡的镇痛作用
Objective To observe the effect of pain hormone C-terminal octapeptide on the analgesic effect of orphaninole against morphine in mice brain. Methods Solid phase peptide synthesis was used to synthesize the octapeptide of acarbose and the pain threshold was determined by radiant heat flick-tail method. (1) The effect of intraventricular (icv) orphanin injection on morphine analgesia was observed. (2) ) Mice intracerebroventricular injection (icv) painful carbon-based octapeptide on the basis of pain threshold in mice; (3) intracerebral ventricular injection of (icv) painful carbon octapeptide and orphanin peptide on morphine analgesia Effect of the effect. Results Orphanin could antagonize the analgesic effect of morphine. The painkiller carbon-terminated octapeptide itself did not affect the basal pain threshold in mice, but reversed the analgesic effect of orphanin on morphine. CONCLUSIONS: The level of spinal-octreotide at the level of the spinal cord can ameliorate the analgesic effect of orphanin against morphine