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为了观察IL 13对肾小球系膜细胞核因子 κB (NF κB )活性的影响 ,探讨IL 13抑制免疫及炎症反应的分子机制。体外培养的大鼠系膜细胞经LPS激活及不同浓度IL 13处理后 ,利用凝胶电泳迁移率试验 (EMSA )检测系膜细胞NF κB的活性。在含 5 %FCS的RPMI 1640培养状态下的肾小球系膜细胞存在一定的NF κB活性 ;LPS激活后系膜细胞NF κB活性显著增高 ;IL 13可抑制基础状态及LPS激活的系膜细胞NF κB活性。结果表明 ,IL 13可抑制体外培养系膜细胞的NF κB活性。从而抑制NF κB参与调控的细胞因子的表达而最终对系膜细胞炎症效应产生调节作用。
In order to observe the effect of IL-13 on the activity of NF-κB in glomerular mesangial cells and to explore the molecular mechanism of IL-13 in suppressing immune and inflammatory responses. The cultured rat mesangial cells were activated by LPS and treated with different concentrations of IL-13. The activity of NF-κB in mesangial cells was detected by electrophoretic mobility shift assay (EMSA). The mesangial cells cultured in RPMI 1640 medium containing 5% FCS had a certain degree of NF-κB activity; NF-κB activity in mesangial cells was significantly increased after LPS activation; IL-13 inhibited basal and LPS-activated mesangial cells NF κB activity. The results showed that IL 13 inhibited the NF κB activity in cultured mesangial cells. Thereby inhibiting the NF κB involved in the regulation of the expression of cytokines and eventually the inflammatory effect of mesangial cells have a regulatory effect.