论文部分内容阅读
目的:探讨氯胺酮对心肌细胞的毒性作用。方法:将经原代培养成活4天后的大鼠心肌细胞分为四组,A组为对照组,B、C、D三组分别加入氯胺酮1×10~(-5)、1×10~(-4)及1×10~(-3)mol/L。实验开始后2、4、8及24小时评定心肌细胞搏动功能、细胞酶及培养液中电解质改变,并观察形态学变化。结果:B组各时点搏动频率均明显加快(P<0.05和P<0.01),C及D组搏动频率减慢(P<0.05和P<0.01),D组在8小时和24小时细胞呈部分搏动或无搏动。随剂量递增及作用时间延长,细胞形态学变化逐渐明显,LDH、AST、CK释放量逐渐增加。结论:氯胺酮浓度为1×10~(-5)mol/L时有正性变时性和变力性作用;浓度≥1×10~(-4)mol/L时,则有明显的抑制作用。
Objective: To investigate the toxic effect of ketamine on cardiomyocytes. Methods: The primary cultured cardiomyocytes were divided into four groups: control group A, control group, group B, C, D were added ketamine 1 × 10 ~ (-5), 1 × 10 ~ ( -4) and 1 × 10 ~ (-3) mol / L. Myocardial cell pulse function was assessed at 2, 4, 8, and 24 hours after the start of the experiment. Electrolytes in the cellular enzyme and culture fluid were changed, and morphological changes were observed. Results: The beating frequency in group B was significantly higher than that in group B (P <0.05 and P <0.01), but the frequency of pulsation in groups C and D was slower (P <0.05 and P <0.01), and in group D at 8 and 24 hours Some beats or no beats. With the increase of dose and the prolongation of action time, the morphological changes of cells gradually became obvious, and the release of LDH, AST and CK increased gradually. CONCLUSION: When chloramine concentration is 1 × 10 ~ (-5) mol / L, there is a positive chronotropic and variable force effect. When the concentration is higher than 1 × 10 -4 mol / L, the inhibitory effect is obvious .