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目的评价异基因造血干细胞(allo-HSCT)联合胸腺移植(TT)时给予去除CD4~+T细胞供体淋巴细胞输注(CD4~--DLI)对T细胞重建及向外周T细胞储存库输出能力的影响。方法分为两组,对照组alloHSCT时联合TT,处理组allo-HSCT时联合TT及CD4~--DLI,移植5个月后提取小鼠脾脏T细胞的基因组DNA,以实时定量PCR检测小鼠脾脏T细胞中信号结合T细胞受体重排删除环(sjTRECs)水平,PCR扩增目的基因与RAG_2片段,建立PCR标准曲线并比较对照组和处理组脾脏T细胞sjTRECs含量。结果对照组和处理组的脾脏T细胞sjTRECs水平差异有统计学意义,处理组显著高于对照组(P<0.01)。结论 allo-HSCT+TT时给予CD4~--DLI能够显著加快移植后T细胞重建及向外周T细胞储存库输出能力的恢复。
Objective To evaluate the effect of CD4 (superscript +) T cell donor lymphocyte infusion (CD4 ~ - DLI) on allogeneic hematopoietic stem cells (allo-HSCT) combined with thymus transplantation (TT) on T cell remodeling and peripheral T cell repertoire The impact of ability. METHODS: The control group was treated with alloHSCT combined with TT, and treated with allo-HSCT combined with TT and CD4 ~ --DLI. The genomic DNA of mouse T lymphocytes was extracted 5 months after transplantation, The spleen T cells were transduced with T cell receptor rearrangement deletion loops (sjTRECs), and the target gene and RAG2 fragments were amplified by PCR. The PCR standard curve was established and the content of sjTRECs in control and treatment groups was compared. Results The levels of sjTRECs in control group and treated group were significantly different from those in control group (P <0.01). Conclusions The administration of CD4 ~ - DLI in allo-HSCT + TT can significantly accelerate the recovery of T cell remodeling and the export of peripheral T cell repertoire after transplantation.