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目的探讨脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)基因多态性与颞叶癫癎的相关性。方法采用提取DNA、聚合酶链反应、测序BDNF基因的66氨基酸外显子rs6265(G/A)多态性,比较颞叶癫癎患者、正常对照组患者基因型频率、等位基因频率差异。结果80例颞叶癫癎患者,AA、GG和GA型分别为24例(30%)、28例(35%)、28例(35%);80名正常人中AA、GG和GA型分别为26例(32.5%)、20例(25%)、34例(42.5%),两组间基因型比较差异无统计学意义(P>0.05)。结论本研究尚未发现颞叶癫癎与BDNF基因rs6265(G/A)多态性存在相关性。
Objective To investigate the relationship between brain-derived neurotrophic factor (BDNF) gene polymorphism and temporal lobe epilepsy. Methods The polymorphisms of 66 amino acid exon rs6265 (G / A) of BDNF gene were extracted by DNA extraction and polymerase chain reaction (PCR). The genotype frequency and allele frequency of patients with temporal lobe epilepsy and normal controls were compared. Results Among 80 patients with temporal lobe epilepsy, AA, GG and GA were 24 (30%), 28 (35%) and 28 (35%) respectively. There were 26 cases (32.5%), 20 cases (25%) and 34 cases (42.5%). There was no significant difference in genotype between the two groups (P> 0.05). Conclusion There is no correlation between temporal lobe epilepsy and rs6265 (G / A) polymorphism of BDNF gene in this study.