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用高效液相色谱分离、测定乌头及附子中一些主要生物碱。生药的乙醚提取物在十八烷基键合相柱上,用甲醇—水—氯仿—三乙胺(70:30:2:0.1)作流动相,中乌头碱、乌头碱及次乌头碱与其它杂质能很好分离。以β-甲基萘作内标化合物,用峰高比测定各生物碱含量。 曾测定了不同品种、不同产地及不同加工炮制方法的一些样品,其生物碱组成及比例相差较大。
Separation and determination of some major alkaloids in Aconitum and Aconite by high performance liquid chromatography. The crude extract of crude drug was on an octadecyl bonded phase column with methanol-water-chloroform-triethylamine (70:30:2:0.1) as mobile phase, mesaconitine, aconitine, and sub-Utah Natrium is well separated from other impurities. Using β-methylnaphthalene as an internal standard compound, the content of each alkaloid was determined using the peak height ratio. Some samples of different varieties, different origins, and different processing methods have been determined and their alkaloid compositions and ratios are quite different.