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目的:研究IFN-γ对卵巢癌耐药细胞株SKOV3TS及敏感细胞株SKOV3中STAT-1表达的调控。方法:应用RT-PCR方法、细胞免疫荧光法检测SKOV3TS与SKOV3在IFN-γ作用前后STAT-1 mRNA及蛋白的表达。用MTT法检测不同浓度IFN-γ作用卵巢癌细胞株不同时间后对细胞增殖率的影响。结果:卵巢癌SKOV3TS、SKOV3细胞株均有STAT-1表达,且主要集中于胞浆。并且随着IFN-γ作用时间延长,STAT-1蛋白表达增强。IFN-γ作用两种细胞株24h及48h后STAT-1 mRNA表达均增加,且差异有统计学意义(P<0.01),但不同浓度IFN-γ作用相同时间后STAT-1 mRNA表达差异无统计学意义(P>0.05)。IFN-γ可显著抑制SKOV3TS和SKOV3细胞增殖,也呈时间依赖性(P<0.01)。IFN-γ对SKOV3组的抑制率较SKOV3TS组高(P<0.05)。结论:IFN-γ能够促进STAT-1表达,抑制卵巢癌细胞增殖。
AIM: To investigate the regulation of STAT-1 expression in ovarian cancer cell line SKOV3TS and in SKOV3 cell line by IFN-γ. Methods: The expression of STAT-1 mRNA and protein in SKOV3TS and SKOV3 cells treated with IFN-γ was detected by RT-PCR and immunofluorescence. The effects of different concentrations of IFN-γ on the proliferation of ovarian cancer cell lines were detected by MTT assay. Results: The expression of STAT-1 in SKOV3TS and SKOV3 cells was mainly in the cytoplasm. And with the prolongation of IFN-γ, STAT-1 protein expression increased. The expression of STAT-1 mRNA in both cell lines increased at 24h and 48h after treatment with IFN-γ, and the difference was statistically significant (P <0.01). However, there was no statistical difference in the expression of STAT-1 mRNA between different concentrations of IFN- Significance (P> 0.05). IFN-γ significantly inhibited the proliferation of SKOV3TS and SKOV3 cells in a time-dependent manner (P <0.01). The inhibitory rate of IFN-γ in SKOV3 group was higher than that in SKOV3TS group (P <0.05). Conclusion: IFN-γ can promote the expression of STAT-1 and inhibit the proliferation of ovarian cancer cells.