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本文采用~3H-TdR掺入法和ConA活化脾细胞检测IL-2的方法观察了松果腺褪黑素(MT)对ConA和LPS诱导小鼠脾T_1,B淋巴细胞增殖反应及ConA诱导大鼠脾淋巴细胞产生IL-2的影响。结果表明,MT0.01～1μmol/L和0.01～10μmol/L分别促进ConA和LPS诱导小鼠脾淋巴细胞增殖反应,ATl～10μmol/L对ConA诱导大鼠脾淋巴细胞产生IL-2亦有促进作用,但MT对未经ConA或LPS活化的淋巴细胞功能则无明显影响,提示丝裂原活化的淋巴细胞可能是MT作用的靶细胞之
In this paper, we observed the effects of melatonin on the proliferation of T 1 and B lymphocytes induced by ConA and LPS, and the effects of ConA on L-2 induced by ConA and LPS in splenocytes detected by 3H-TdR incorporation and ConA-activated spleen cells The Effect of IL-2 Production by Splenic Lymphocytes in Mice. The results showed that MT1.01 ~ 1μmol / L and 0.01 ~ 10μmol / L promoted the proliferation of splenic lymphocytes induced by ConA and LPS, respectively. AT1 ~ 10μmol / L promoted the production of IL-2 by ConA in splenic lymphocytes However, MT had no significant effect on lymphocyte function without ConA or LPS activation, suggesting that mitogen-activated lymphocytes may be target cells of MT effect