论文部分内容阅读
目的 为了通过细胞融合研制肺癌疫苗，需要建立有适合细胞融合的肺癌抗原细胞。方法 用８氮鸟嘌呤（８ａｚａｇｕａｎｉｎｅ，８ Ａ Ｇ）从小鼠肺癌细胞 Ｌ Ａ７９５ 诱导抗８ Ａ Ｇ 细胞株 Ａ Ｌ Ａ９７０２ ，并鉴定其生物学特性及成瘤性。结果 Ａ Ｌ Ａ９７０２ 细胞在１．３１×１０－ ４ｍ ｏｌ／ Ｌ ８ Ａ Ｇ 培养液中生长了１ 年，在 Ｈ Ａ Ｔ培养液中不能形成集落，并保持了来源细胞 Ｌ Ａ７９５ 的生长特性、染色体数目、成瘤性及肺转移性。 结论 Ａ Ｌ Ａ９７０２细胞保持了来源细胞的生物学性质及成瘤性质，是一株稳定的抗８ Ａ Ｇ的小鼠肺癌细胞株，将作为适合细胞融合的抗原细胞，为研制肺癌疫苗，建立对肺癌有效的特异性免疫治疗奠定良好的基础。
Objective To develop a lung cancer vaccine by cell fusion, lung cancer antigen cells suitable for cell fusion need to be established. Methods A L A9702 cells were induced by 8azaguanine (8A G) from mouse lung cancer cell line L A795 and their biological characteristics and tumorigenicity were identified. Results A L A9702 cells grew for 1 year in 1.31×10-4 mol/L 8A G medium. Colonies could not be formed in H A T culture medium, and the growth characteristics of L A795 cells were preserved. Chromosomal number, tumorigenicity, and metastatic lung. Conclusion A L A9702 cell maintains the biological properties and tumorigenicity of the source cells. It is a stable anti-8-A G mouse lung cancer cell line, which will be used as an antigen-adapted cell fusion cell for the development of a lung cancer vaccine. It lays a good foundation for effective specific immunotherapy for lung cancer.