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在室温下，用ＵＶ－２１００型紫外可见分光光度计考察各种ｐＨ值的缓冲液对Ｆｅ＿４Ｓ＿４簇合物中μ＿３－Ｓ的破坏程度，藉此考察ＦｅＭｏ－ｃｏ中μ＿３－Ｓ的酸不稳定性．结果表明Ｆｅ＿４Ｓ＿４簇合物中的μ＿３－Ｓ在ｐＨ２～３时最不稳定，极易受酸破坏；ｐＨ３～４时，Ｆｅ＿４Ｓ＿４簇中的μ＿３－Ｓ受酸影响较ｐＨ２～３时小；ｐＨ４～５时，Ｆｅ＿４Ｓ＿４簇基本稳定，其中的μ＿３－Ｓ基本不受破坏．结合蛋白环境对金属中心有巨大保护作用，在生物提取ＦｅＭｏ－ｃｏ的过程中酸解对蛋白键合的ＦｅＭｏ－ｃｏ骨架μ＿３－Ｓ的破坏是很微弱的，但对裸露的或外围蛋白受到破坏的ＦｅＭｏ－ｃｏ骨架μ＿３－Ｓ的破坏却是相当大的．
At room temperature, the UV-2100 UV-Vis spectrophotometer was used to investigate the damage of μ_3-S in the Fe_4S_4 cluster by various pH buffers to investigate the acid-labile μ_3-S in FeMo-co . The results showed that μ_3-S in Fe_4S_4 cluster was the most unstable at pH 2 ~ 3 and was easily damaged by acid. At pH 3 ~ 4, μ_3-S in Fe_4S_4 cluster was less affected by acid than pH 2 ~ 3, 5, the Fe_4S_4 clusters were basically stable, among which μ_3-S was not destroyed basically. The binding protein environment has a great protective effect on the metal center. The destruction of protein-bound Fe 3 O-Co skeleton μ_3-S by acid hydrolysis during the biological extraction of FeMo-co is very weak, but the bare or peripheral protein is destroyed The destruction of μ_3-S in the FeMo-co framework is quite large.