Ｔ淋巴细胞活化是一个涉及多种膜表面分子和受体以及一系列相关多肽的复杂过程，为了使Ｔ细胞发挥更好的识别和杀伤癌细胞的功能，采用抗ＣＤ３、ＣＤ２８、ＣＤ８０（Ｂ７１）、ＣＤ２、ＣＤ５８ＭｃＡｂ分别刺激健康人ＰＢＬｓ后作用肝癌细胞，对作用前后ＰＢＬｓ用ＦＡＣＳ进行表型分析，结果发现：作用后ＣＤ３和ＣＤ８分子表达比作用前明显增高，而ＣＤ４分子无显著变化，同时基因家族采用ＲＴＰＣＲＳｏｕｔｈｅｒｎ印迹分析ＴＣＲＶβ基因１～２０亚家族表达水平与特征，健康人ＰＢＬｓ分别加入ＩＬ２、ＰＨＡ、抗ＣＤ３和ＣＤ３＋ＣＤ２８、ＣＤ２８＋ＣＤ８０、ＣＤ２＋ＣＤ５８作用肝癌细胞（ＢＥＬ７４０２）前表达水平平均约为５％，作用ＢＥＬ７４０２后表达水平约为１３％～２５％，其特征为Ｖβ７增高，这提示在癌抗原的参与下ＭｃＡｂ共刺激的Ｔ细胞活化，ＴＣＲ接受ＡＰＣ相应抗原的刺激，具有该ＴＣＲ的淋巴细胞迅速增殖而成为针对抗原的Ｔ细胞克隆，发挥其识别和杀伤癌细胞的作用，这将为肿瘤生物治疗的研究提供分子免疫学依据。 T-lymphocyte activation is a complex process involving various membrane surface molecules and receptors and a series of related polypeptides. In order to enable T cells to better recognize and kill cancer cell functions, anti-CD3, CD28, and CD80 (B7) are used. 1) CD2 and CD58 McAb stimulated PBLs in healthy humans and acted on hepatocarcinoma cells. PBLs were subjected to phenotypic analysis before and after treatment. The results showed that the expression of CD3 and CD8 molecules was significantly higher than before, and there was no significant change in CD4 molecules. At the same time, RTPCRSouthern blotting analysis was used to analyze the expression levels and characteristics of TCRVβ gene sub-family 1 to 20 subgroups. Healthy human PBLs were added with IL2, PHA, anti-CD3 and CD3+CD28, CD28+CD80, and CD2+CD58 for hepatoma cells (BEL7402). The average expression level before the test was approximately 5%, and the expression level after the action of BEL-7402 was approximately 13% to 25%. The increase of β7 suggests that the McAb co-stimulated T cells were activated under the participation of cancer antigens, TCR was stimulated by the corresponding antigens of APC, and lymphocytes with this TCR rapidly expanded to become T cell clones against the antigen, exerting their functions of recognizing and killing cancers. The role of cells, which will provide molecular immunological basis for the study of cancer biological therapy.