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在重症肝炎病人血清中发现的人肝细胞增殖因子(human hepatocyte growth factor hHGF)就是当初提纯的人肝细胞增殖因子(HGF)。从大白鼠血小板或家兔血清中也提纯出有类似生化学性状的 HGF,经 cDNA 克隆或部分氨基酸序列分析,证实这些因子均属于同一 HGF 族。一、hHGF 的性质和构造从重症肝炎病人血清提纯 hHGF 用 SDS-PAGE分析,证实其分子量约为9万,是由分子量6万的重链和分子量3万的轻链,经双硫键结合而形成的异二聚体。因为对原代培养的大白鼠肝细胞的 DNA 合成,它同适当浓度的胰岛素或上皮生长因子(EGF)有相加或相乘的促进活性,所以认为它们是通过不同受体起作用的。迄今为止,有关 HGF 的受体研究甚少,最近有二个重要的报告,①c-met 癌基因表达产物可能就是 HGF 的受体;②肝素结合性 HGF 能促进细胞膜上的145kd 蛋白质的磷酸化。
The human hepatocyte growth factor (hHGF) found in sera from patients with severe hepatitis is the original purified human hepatocyte growth factor (HGF). HGF with similar biochemical traits was also purified from rat platelets or from rabbit serum. All of these factors were confirmed to belong to the same HGF group by cDNA cloning or partial amino acid sequence analysis. First, the nature and structure of hHGF Purified hHGF serum from patients with severe hepatitis by SDS-PAGE analysis, confirmed its molecular weight of about 90,000, is composed of a heavy chain of molecular weight of 60,000 and a molecular weight of 30,000 light chain, the combination of disulfide bond The formation of heterodimers. Because of the DNA synthesis of primary cultured rat hepatocytes that has additive or multiplicative promotive activity with appropriate concentrations of insulin or epidermal growth factor (EGF), they are thought to act through different receptors. So far, few reports have been reported on HGF receptors. Recently, there are two important reports. (1) The expression of c-met oncogene may be the receptor of HGF. (2) Heparin-binding HGF can promote the phosphorylation of 145 kd protein on the cell membrane.