乙二醇法是在动物身上复制泌尿系统结石模型最常用的方法，也是新药评审中规定选用的方法之一［１］。该方法的原理是在草酸代谢通路上加大中间代谢产物乙二醇的量，导致动物体内草酸含量增加而形成草酸钙结石［２］。因此法操作简单，成石率高，已被广泛应用于防治泌尿系结石 Ethylene glycol method is the most commonly used method for replicating urinary calculi models in animals and is also one of the methods used in the evaluation of new drugs [1]. The principle of this method is to increase the amount of ethylene glycol, an intermediate metabolite, in the pathway of oxalic acid metabolism, resulting in the formation of calcium oxalate stones in oxalic acid in animals [2]. Therefore, the method is simple, into a high rate of stone, has been widely used in prevention and treatment of urinary stones