论文部分内容阅读
目的研究多发性肌炎(PM)患者肌肉组织中CD28分子的表达情况,探讨CD28在PM中的作用。方法临床和病理诊断确诊的PM患者17例,对照组为7例肌营养不良患者。采用免疫组织化学方法和实时荧光定量PCR方法检测肌肉中CD28的表达水平。结果与肌营养不良患者比较,免疫组织化学方法观察到PM患者肌肉组织中CD28表达量减少;实时荧光定量PCR结果两组之间CD28mRNA表达有统计学差异,PM患者表达降低(P<0.01)。结论 CD28的减少参与多发性肌炎的病理过程。
Objective To study the expression of CD28 in muscle tissue of patients with polymyositis (PM) and to explore the role of CD28 in PM. Methods Seventeen patients with PM were diagnosed clinically and pathologically. The control group was 7 patients with muscular dystrophy. The expression of CD28 in muscle was detected by immunohistochemistry and real-time fluorescence quantitative PCR. Results Compared with patients with muscular dystrophy, the expression of CD28 in muscle tissue of PM patients was decreased by immunohistochemistry. The results of real - time fluorescence quantitative PCR showed that CD28 mRNA expression was significantly different between the two groups (P <0.01). Conclusions The decrease of CD28 is involved in the pathological process of polymyositis.