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由青环海蛇(Hydrophis Cyanocinctus)提取得青环海蛇毒 G-5-C-11组分、经小鼠尾静脉注射蛇毒,其 LD50为0.346±0.09mg/kg,对猫静脉滴注蛇毒0.7～1.04mg/kg 时,使呼吸停止、血压下降,在人工呼吸维持下,血压、心率、心电图可回复到给毒前水平,连续给蛇毒3小时,当剂量为3.4～4mg/kg 时,均未见血压,心率的改变。在离体豚鼠心脏灌流实验中,即使剂量加大到1mg/0.1ml,心肌收缩力、心率、冠脉流量亦无显著差异,蛇毒浓度为10~(-6)mg/ml 时,能使小鸡颈二腹肌神经肌肉出现不可逆性的神经—肌肉传递阻滞,当加大浓度到2×10~(-6)g/ml 时,还出现肌肉收缩力明显减弱的毒性作用,本实验结果表明青环海蛇蛇毒 G-5-C-11组分主要是神经毒作用,还对横纹肌有一定的毒性影响。
The Gynostemma pentaphylla G-5-C-11 fraction was extracted from Hydrophis Cyanocinctus, and the LD50 was 0.346 ± 0.09 mg / kg via the tail vein of mice injected intravenously with a dose of 0.7 ~ 1.04mg / kg, the respiration stopped, blood pressure decreased, maintained at artificial respiration, blood pressure, heart rate, ECG can be restored to the level before giving poison, continuous to snake venom 3 hours, when the dose of 3.4 ~ 4mg / kg, none See blood pressure, heart rate changes. In the guinea pig heart perfusion experiment, there was no significant difference in myocardial contractility, heart rate and coronary flow even if the dose was increased to 1mg / 0.1ml. When the venom concentration was 10 ~ (-6) mg / ml, The muscle-muscle block of the neuromuscular muscles of the chicken biceps muscle appeared irreversible. When the concentration was increased to 2 × 10 -6 (-6) g / ml, the contractile force of the muscles was obviously weakened. The results of this experiment It showed that the components of G-5-C-11 in Qinghai Sea snake venom were mainly neurotoxic and also had some toxic effects on striated muscle.