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目的检测自然流产患者中的HPV B19 Ig M、Ig G抗体及DNA,探讨其对自然流产的预防及诊治价值。方法收集病例组(自然流产孕妇)和对照组(中晚期正常妊娠孕妇)静脉血,采用ELISA法检测HPV B19 Ig M、Ig G抗体,PCR检测HPV B19 DNA,比较自然流产患者与正常待产孕妇感染率的差异。结果病例组检测到3例HPV B19 Ig M阳性标本,对照组未检测到阳性标本;病例组HPV B19 Ig G阳性率为46.15%,低于对照组阳性率63.46%;病例组HPV B19 DNA阳性率为21.15%,显著高于对照组阳性率3.85%。两组差异有统计学意义(P<0.05)。结论长沙地区自然流产孕妇中HPV B19的感染率高于正常妊娠孕妇,推测HPV B19感染与自然流产有相关性。
Objective To detect HPV B19 Ig M and Ig G antibodies and DNA in patients with spontaneous abortion, and to explore its value in prevention and diagnosis of spontaneous abortion. Methods Venous blood was collected from case group (spontaneous abortion pregnant women) and control group (middle and late normal pregnant women). ELISA was used to detect HPV B19 Ig M and Ig G antibodies. PCR was used to detect HPV B19 DNA. The spontaneous abortion patients were compared with normal pregnant women Rate difference. Results Three cases of HPV B19 Ig M positive specimens were detected in the case group and no positive specimens were detected in the control group. The positive rate of HPV B19 Ig G was 46.15% in the case group, which was lower than that in the control group (63.46%). The positive rate of HPV B19 DNA in the case group 21.15%, significantly higher than the control group positive rate of 3.85%. The difference between the two groups was statistically significant (P <0.05). Conclusion The prevalence of HPV B19 in spontaneous abortion pregnant women in Changsha area is higher than that in normal pregnant women. It is speculated that HPV B19 infection is associated with spontaneous abortion.