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目的对产类细菌素嗜酸乳杆菌P302的发酵条件进行研究。方法采用琼脂扩散法测定发酵上清液对大肠埃希菌O139的抑菌活性。结果通过正交试验确定P302产类细菌素的最佳培养基组分为牛肉膏4%、胰蛋白胨0.2%、酵母粉0.8%、碳酸钙0.4%和蔗糖1%,0.4%复合维生素,0.3%Tween-80有利于类细菌素的产生。发酵工艺条件为最适初始pH7.0,最适温度37℃,最佳接种量0.3%,种龄14h,厌氧培养22h。结论通过优化发酵条件和发酵工艺,类细菌素的产量提高了35%。
Objective To study the fermentation conditions of the bacterium Lactobacillus acidophilus P302. Methods The antibacterial activity of fermentation supernatant against Escherichia coli O139 was determined by agar diffusion method. Results The optimal culture medium composition of P302 bacteriocin production was determined by orthogonal test. The optimum culture medium was 4% beef extract, tryptone 0.2%, yeast extract 0.8%, calcium carbonate 0.4% and sucrose 1%, 0.4% multivitamin, 0.3% Tween-80 is good for bacterioides production. The optimum fermentation conditions were initial pH7.0, optimal temperature 37 ℃, optimal inoculation 0.3%, seedling age 14h, anaerobic culture 22h. Conclusions Bacteriocin production increased by 35% by optimizing fermentation conditions and fermentation processes.