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目的:建立HPLC-ELSD法测定三七血伤宁胶囊中三七皂苷R_1、人参皂苷Rg_1和人参皂苷Rb_1的含量。方法:采用HYPERSIL C_(18)柱(4.6 mm×250 mm,5μm)色谱柱,以乙腈(A)-水(B)为流动相进行梯度洗脱[0～25 min,A-B(20:80);25～75 min,A-B(20:80)→A-B(35:65)],流速1.0 mL·min~(-1),柱温35℃,漂移管温度106℃,气体流速2.9 L·min~(-1)。结果:三七皂苷R_1在0.225～5.62μg范围内线性关系良好(r=1.000),平均加样回收率(n=9)为101.3%;人参皂苷Rg_1在2.08～20.8μg范围内线性关系良好(r=0.9997),平均加样回收率(n=9)为99.9%;人参皂苷Rb_1在2.06～20.6μg范围内线性关系良好(r=0.9998),平均加样回收率(n=9)为96.9%。结论:本方法操作简便,结果准确,灵敏度高,重现性好,可作为该产品质量控制的方法。
Objective: To establish a HPLC-ELSD method for the determination of notoginsenoside R_1, ginsenoside Rg_1 and ginsenoside Rb_1 in Sanqi Xuetaning capsule. METHODS: The gradient elution was performed using acetonitrile (A)-water (B) as the mobile phase on a HYPERSIL C 18 column (4.6 mm×250 mm, 5 μm) column. [0–25 min, AB(20:80) 25-75 min, AB(20:80)→AB(35:65)], flow rate 1.0 mL·min-1, column temperature 35°C, drift tube temperature 106°C, gas flow rate 2.9 L·min~ (-1). RESULTS: The linear relationship of notoginsenoside R 1 in the range of 0.225-5.62 μg was good (r=1.000), the average recovery (n=9) was 101.3%, and the ginsenoside Rg-1 had good linearity in the range of 2.08-20.8 μg ( r=0.9997). The average recovery (n=9) was 99.9%. The ginsenoside Rb_1 had a good linear relationship (r=0.9998) in the range of 2.06 to 20.6 μg. The average recovery (n=9) was 96.9. %. Conclusion: This method is simple, accurate, sensitive, and reproducible. It can be used as a method for quality control of the product.