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为了探讨“脾主肌肉”的机理，利用脾气虚的模型动物，测定肌肉、血液中与肌肉能量代谢物质代谢有关酶活性的变化，从这些指标的变化来了解脾与肌肉的关系。脾气虚模型动物制作是依据我所已建立的模型。通过实验发现，脾气虚模型动物肌肉中能量代谢和物质代谢水平明显低于正常组。肌肉抽提液内源性ＡＴＰ（三磷酸腺苷）的含量，磷酸肌酸激酶活性，这些与能量代谢有关的指标，脾气虚组明显低于正常组。而与肌肉物质代谢有关的酶：乳酸脱氧酶、谷丙转氨酶、α－羟丁酸脱氢酶等酶的活性，脾气虚组也明显低于正常组。根据脾气虚模型动物实验表明：脾气虚，机体运化功能不足，肌肉中能量供应不足，物质合成低下，造成肌瘦无力。该实验方法为更深入地探讨“脾主肌肉”机理开创一条新路。
In order to explore the mechanism of “spleen and main muscle”, the model animals with spleen deficiency were used to determine the changes of enzyme activities related to the metabolism of energy metabolites in muscle and blood. The changes of these indexes were used to understand the relationship between spleen and muscle. Spleen qi model animal production is based on the model I have established. Through experiments, it is found that the energy metabolism and the level of substance metabolism in the muscle of spleen deficiency model animal are obviously lower than those in the normal group. Muscle extract of endogenous ATP (adenosine triphosphate) content, creatine phosphokinase activity, these energy metabolism-related indicators, spleen deficiency group was significantly lower than the normal group. However, the activities of enzymes related to muscle metabolism, such as lactate dehydrogenase, alanine aminotransferase, and a-hydroxybutyrate dehydrogenase, were also significantly lower in the spleen deficiency group than in the normal group. According to the spleen qi deficiency model animal experiments show that: spleen deficiency, lack of transport function of the body, lack of energy supply in the muscle, the material synthesis is low, resulting in weakness. This experimental method opens a new path for exploring the mechanism of “spleen and muscle” in more depth.