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目的：应用研究高功率微波对金黄葡萄球菌25932的杀灭效果及其可能存在的非热效应。方法：采用频率2450MHz，功率0～3kW的连续高功率微波，对于湿状态的细菌进行不同强度及时间的辐照，比较其杀灭率，并同时与单纯的加热灭菌相对比，研究高功率微波对它的杀灭规律。结果：不同强度及辐照时间的微波对干菌作用后，其温度无显著变化，但活菌数显著减少（P＜0．05）；对湿菌的杀灭效果则显著高于干菌。而单纯加热灭菌率则低于微波对湿菌的杀灭。结论：微波对湿菌的杀灭强于单纯的加热灭菌，且可杀灭干菌．说明微波对细菌的杀灭除热效应外还有非热效应。
Objective: To study the killing effect of high power microwave on Staphylococcus aureus 25932 and its possible non-thermal effect. Methods: Using continuous high-power microwave with frequency of 2450MHz and power of 0 ~ 3kW, the bacteria in wet state were irradiated with different intensity and time, and their killing rates were compared. Meanwhile, compared with simple heat sterilization, Microwave kill it on the law. Results: The microwaves with different intensities and irradiation time showed no significant changes in temperature, but the number of viable cells decreased significantly (P <0.05). The killing effect on wet bacteria was significantly higher than that of dry bacteria. The simple heat sterilization rate is lower than the microwave kill wet bacteria. Conclusion: Microwave sterilization of wet bacteria is stronger than simple heat sterilization, and can kill dry bacteria. Describe the killing of bacteria in addition to microwave heat effects in addition to non-thermal effects.